期刊论文

EFfect of cooking oil fumes on cell cycle and apoptosis in lung cells of spraque dawley rats

中文

中国公共卫生

1001-0580

2004

20

6

714-715

10.3321/j.issn:1001-0580.2004.06.042

本校为第一机构

目的检测SD(Spraque dawley)大鼠吸入烹调油烟(cooking oil fumes,COF)后肺组织细胞周期和凋亡的变化.方法以SD大鼠作为实验对象,通过动式吸入染毒,于染毒后第11个月末,采用流式细胞仪(flow cytometer,FCM)技术检测肺组织的细胞周期和凋亡.结果 G1期细胞所占百分数随染毒剂量的增加而减少,各组间的G1%有显著性差异(F=5.08,P＜0.05);S期细胞所占百分数随染毒剂量的增加而增高,组间S%有显著性差异(F=17.06,P＜0.01).各组间肺组织细胞凋亡率有显著性差异(X2=51.17,P＜0.01),且存在剂量-反应关系(r=0.805,P＜0.05).结论在细胞周期中,G1期细胞所占百分数随染毒剂量的增加而减少,S期细胞所占百分数随染...

Objective To detect the lung tissue cell cycle and apoptosis of spraque dawley(SD) rats after inhaling cooking oil fumes(COF).Methods SD rats were used to make the chronic toxicity model.Experiment groups were exposed to COF of three kinds of different concentration(6.88±0.31)?(15.06±0.35)?(35.33±1.69)mg/m 3 171 times in 11 months,30min per time.Control group had inhaled clean air.Flow cytometer technique was used to detect lung tissue cell cycle and apoptosis.Results Lung tissue cell apoptosis rate decreased with the rise of COF concentrati...