期刊论文

中文

中国公共卫生

1001-0580

2004

20

1

13-15

10.3321/j.issn:1001-0580.2004.01.009

国家自然科学基金重点项目 （ 30 0 30 1 2 0 ）；

本校为其他机构

目的研究4-壬基酚(4-NP)在体外条件下对大鼠胚胎生长发育的影响,探讨其胚胎毒性作用机制.方法采用植入后全胚胎培养模型,将9.5 d Wistar大鼠胚胎与含4-NP的大鼠即刻离心血清共培养48 h(4-NP终浓度为0,6.25,12.5,25,50,100 mg/L),观察4-NP对大鼠胚胎生长发育和组织器官形态分化的影响,并用光镜、电镜检测胚胎和卵黄囊的组织结构.结果 4-NP在25 mg/L以上可诱发卵黄囊生长和血管分化不良、胚胎生长迟缓及器官形态分化异常,严重者出现神经系统、心脏、腮弓发育异常及小肢芽、体位异常等畸形;100 mg/L的4-NP对体外培养胚胎主要呈致死效应.光镜可见实验组胚胎多个器官组织出现病理改变,光镜和电镜下可...

Objective To investigate the effects of 4-nonylphenol (4 -NP) on cultured rat embryos in vitro and to explore its embryonic toxic mec hanisms.MethodsThe 9.5-day-old Wistar rat embryos were explanted and cultured at six different concentrations of 4-NP in rat immediately centrif ugal serum(0,6.25,12.5,25,50,100?mg/L) for 48 hours.Light microscope and electr onic miroscope were used to observe structure of cultured embyos and their visc eral yolk sacs (VYS).Results4-NP at higher level(≥25?mg/L) could inhibit th e growth and cardiac tube differen...