期刊论文

中文

中华病理学杂志

0529-5807

2002

31

3

240-244

10.3760/j.issn:0529-5807.2002.03.011

国家自然科学基金资助项目 （3 9770 3 71）；

本校为其他机构

目的探讨巨噬细胞集落刺激因子受体(M-CSF-R)在人白血病细胞系中的表达及其作用.方法采用ABC免疫酶标技术、间接免疫荧光染色、流式细胞计数和蛋白免疫印迹研究了4株人白血病细胞系(J6-1、J6-2来源于人粒-单型白血病,HL-60为来源于人急性粒细胞白血病的髓样细胞系, K562为来源于人慢性白血病急变期胸水的髓样细胞系)和正常人外周血单个核细胞及正常人脐带血单个核细胞M-CSF-R的分布、表达、分子大小及其作用.结果正常人外周血单个核细胞未见M-CSF-R的表达,经PHA刺激后有低水平的表达;4株人白血病细胞系都高表达M-CSF-R,分布于细胞膜、细胞质和细胞核;J6-1、J6-2、K562和HL-60细胞质和胞核M-CSF-...

OBJECTIVE: To explore the expression, characteristics and roles of macrophage colony-stimulating factor receptor (M-CSF-R) in human leukemia cell lines. METHODS: Peripheral blood mononuclear cells (PBMCs) collected from 3 healthy persons, cord blood mononuclear cells (CBMCs) collected from 5 healthy persons and 4 human myelomonocytic leukemia cell lines including J6-1, J6-2, K562 and HL-60 were studied by using ABC immunoperoxidaes assay, indirect immunofluorescene staining, flow cytometry, and Western blot. RESULTS: M-CSF-R was noticed to be localized in the cytoplasm, nucleus and at the memb...