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Visualizing endoplasmic reticulum carboxylesterase activity during heat shock by a near-infrared fluorescent probe

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成果类型:
期刊论文
作者:
Li, Songjiao*;Liu, Ying;Deng, Min;Zeng, Jiayu;Cheng, Dan;...
通讯作者:
Li, Songjiao;He, LW;Cheng, D
作者机构:
[He, LW; Liu, Ying; Deng, Min; Zeng, Jiayu; Li, Songjiao; He, Longwei] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421002, Peoples R China.
[Cheng, Dan] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Hengyang Med Sch, Hengyang 421002, Peoples R China.
通讯机构:
[He, LW ; Li, SJ; Cheng, D ] U
Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421002, Peoples R China.
Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Hengyang Med Sch, Hengyang 421002, Peoples R China.
语种:
英文
关键词:
Fluorescent probe;Endoplasmic reticulum;Carboxylesterase;Heat stroke
期刊:
Dyes and Pigments
ISSN:
0143-7208
年:
2024
卷:
223
页码:
111823
基金类别:
Natural Science Foundation of Hunan Province [2022JJ30484, 2022JJ10042, 2022JJ40358]; University of South China [201RGC012, 20XQD030]; Research Foundation of Education Bureau of Hunan Province, China [22B0403]; Open Fund of State Key Laboratory of Chemo/Bio- sensing and Chemometrics [2021013, 2021015]
机构署名:
本校为第一且通讯机构
院系归属:
医学院
摘要:
In heat shock, various enzyme activities are clearly altered due to high body temperature. Evidence accumulated in the last few years indicates that endogenous carboxylesterase (CE) can be regarded as a biomarker of heat shock due to its close relationship to heat shock. To monitor CE activity, this study aims to develop excellent fluorescent probe for CE detection. Fluorescent probe DCM-Br-CE with a NIR turn-on signal at 660 nm and a large Stokes shift of 160 nm, as well as high sensitivity (0.117 x 10-4 U/mL) and good detection capability (30 min) for CE. DCM-Br-CE also has excellent subcell...

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