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A novel detection of radon based on its decay product inducing conformational changes of an aptamer probe

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成果类型:
期刊论文
作者:
Long, Minzhi;Deng, Han;Tian, Gang;Song, Chunli;Liu, Hongwen;...
通讯作者:
Lv, Changyin
作者机构:
[Long, Minzhi; Deng, Han; Shen, Yi; Lv, Changyin; Liu, Hongwen; Tian, Gang; Song, Chunli] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构:
[Lv, Changyin] U
Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
Biosensor;Fluorescence;G-quadruplex;Label-free;Lead ion;Radon
期刊:
Analytica Chimica Acta
ISSN:
0003-2670
年:
2016
卷:
936
页码:
202-207
基金类别:
The authors gratefully acknowledge the support of the National Natural Science Foundation of China (No. 81473021 ).
机构署名:
本校为第一且通讯机构
院系归属:
公共卫生学院
摘要:
This study proposes a novel method for the detection of inert gas radon using a label-free, specific, fluorescence-sensing aptamer in the context of PW17-OG system. This method utilizes the cyanine dye OliGreen (OG) as a signal reactor and the aptamer PW17 as a fluorescent identification probe. When OG integrates into the free curling PW17, a strong fluorescence signal is generated. After radon decays, the long lived naturally occurring radon progeny Pb being disposed and introduced to the system. Lead ions induce PW17 to form a stable G-quadruplex, thereby inhibiting the interaction between O...

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