期刊论文

中文

中华放射医学与防护杂志

0254-5098

2016

36

5

395-400

10.3760/cma.j.issn.0254-5098.2016.05.020

国家自然科学基金委员会-中国科学院大科学装置科学研究联合基金

本校为第一机构

目的以结肠癌细胞HCT116为研究对象,评价DNA依赖蛋白激酶催化亚基(DNA-PKcs)抑制剂NU7026对癌干细胞的放射增敏作用及其机制。方法以CD133 +/CD44 +为标志物,流式细胞术检测癌干细胞亚群。将HCT116细胞分为对照组、单纯药物(20 μmol/L NU7026)组、单纯照射(2 Gy γ射线)组和药物+照射(20 μmol/L NU7026联合2 Gy γ射线)组,照射前2 h加入NU7026。细胞集落形成实验检测HCT116细胞增殖,流式细胞术检测细胞周期和凋亡,γ-H2AX foci的免疫荧光激光共聚焦分析DNA双链断裂损伤修复。结果体外培养HCT116细胞中CD133 +/CD44+癌干细胞亚群比例高达(88.14 ±0.47)%,HCT116细胞低密度接种无血清培养基中集...

Objective To evaluate the radiosensitization and mechanism of DNA-dependent protein kinase catalytic subunit inhibitor NU7026 on HCT1 16 colorectal cancer stem cells. Methods The flow cytometry was used to determine the sub-population of cancer stem cells with the markers CD 133/ CD44. The cells were divided into four groups: control group, 20 μmol/L NU7926 group, 2 Gy irradiation group, and 20 μmol/L NU7026 combined with 2 Gy irradiation group. Cell proliferation and survival were evaluated by colony-formation experiment. Flow cytometry was ...