Objective To explore the expression changes of SIK2 in both protein and mRNA levels in response to ionizing radiation and its potential relationship with the cell cycle distribution alteration. Methods HepG2 cells were irradiated with 6~Co ~/-rays. Western blot and Real-time PCR were used to detect the level of the protein and mRNA, respectively. TdR double-blocking method was used to synchronize cell and the cell cycle distribution was analyzed by flow cytometry. Results The protein level of SIK2 was significantly increased at 4, 6, and 10 h （...
