期刊论文

中文

理化检验-化学分册

1001-4020

2009

45

10

1194-1196

湖南省自然科学基金资助课题（03JJY3030）；

本校为第一机构

在pH 8.0的B-R缓冲溶液中,加入适量表面活性剂Triton X-100,可引起1-羟基芘的荧光显著增强,在3.2～982.0 μg·L-1范围内荧光强度与1-羟基芘的质量浓度呈线性关系.方法的检出限(3S/N)为1.0 μg·L-1.据此,提出了测定人尿中1-羟基芘的新方法.方法的加标回收率为93.2%～100.7%范围,对200.0,600.0 μg·L-1 1-羟基芘标准溶液平行测定7次,相对标准偏差分别为1.78%和0.98%.应用于人尿中痕量1-羟基芘的测定,结果与高效液相色谱法一致.

It was found that in a B-R buffer medium of pH8.0, the fluorescence intensity of 1-hydroxypyrene （1-OHP in short） was significantly sensitized by the Triton X-100 micelle, and linear relationship was obtained between values of fluorescence intensity and mass concentration of 1-OHP in the range from 3. 2 to 982. 0 μg · L^-1 when measured at the wavelengths of （λex） 279 nm and （λem） 390 nm Detection limit （3S/N） of the method found was 1.0μg ·L^-1. Based on these facts, a method for determination of 1-OHP in human urine was proposed...