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Protective effects of dexmedetomidine on hypoxia/reoxygenation injury in cardiomyocytes by regulating the CHOP signaling pathway

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成果类型:
期刊论文
作者:
Shi, Xiaoqiao;Liu, Zhiwen;Li, Junwei
通讯作者:
Li, JW
作者机构:
[Shi, Xiaoqiao; Liu, Zhiwen] Univ South China, Affiliated Hosp 2, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.
[Li, Junwei] Hunan Univ Tradit Chinese Med, Dept Anesthesiol, Affiliated Hosp 2, 233 North Caie Rd, Changsha 410005, Hunan, Peoples R China.
通讯机构:
[Li, JW ] H
Hunan Univ Tradit Chinese Med, Dept Anesthesiol, Affiliated Hosp 2, 233 North Caie Rd, Changsha 410005, Hunan, Peoples R China.
语种:
英文
关键词:
CCAAT enhancer binding protein;CCAAT enhancer binding protein homologous protein;dexmedetomidine;glucose regulated protein 78;interleukin 1beta;interleukin 6;lactate dehydrogenase;malonaldehyde;nitric oxide;superoxide dismutase;tumor necrosis factor;unclassified drug;antiinflammatory agent;Ddit3 protein, rat;dexmedetomidine;growth arrest and DNA damage inducible protein 153;lactate dehydrogenase;apoptosis;Article;cell viability;colorimetry;controlled study;enzyme activity;flow cytometry;gene knockdown;gene overexpression;gene silencing;H9c2(2-1) cell line;heart protection;mRNA expression level;MTT assay;myocardial ischemia reperfusion injury;protein expression level;real time reverse transcription polymerase chain reaction;signal transduction;Western blotting;animal;biological model;cardiac muscle cell;cell hypoxia;cell line;cell proliferation;cell survival;cytology;drug effect;endoplasmic reticulum stress;gene expression regulation;genetics;metabolism;signal transduction;Animals;Anti-Inflammatory Agents;Cell Hypoxia;Cell Line;Cell Proliferation;Cell Survival;Dexmedetomidine;Endoplasmic Reticulum Stress;Gene Expression Regulation;L-Lactate Dehydrogenase;Models, Biological;Myocytes, Cardiac;Signal Transduction;Transcription Factor CHOP
期刊:
MOLECULAR MEDICINE REPORTS
ISSN:
1791-2997
年:
2020
卷:
22
期:
4
页码:
3307-3315
机构署名:
本校为第一机构
院系归属:
医学院
摘要:
Hypoxia/reoxygenation (H/r) injury in myocardial cells occurs frequently during cardiac surgery and affects the prognosis of patients. The present study aimed to investigate the protective effects of dexmedetomidine (dex) on H/r injury and its association with the c/eBP-homologous protein (cHoP) signaling pathway. an H/r model was constructed in H9c2 cells to investigate the effects of dex on H/r injury. cell viability, apoptosis and lactate dehydrogenase (LDH) levels were determined by MTT, flow cytometry and 2,4-dinitrophenylhydrazine colorim...

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