期刊论文

中文

热带病与寄生虫学

1672-2302

2004

2

1

16-18

10.3969/j.issn.1672-2302.2004.01.005

湖南省教育厅科研基金资助（课题编号:4-02-JY-02-C388）；

本校为第一机构

目的构建日本血吸虫中国大陆株粘蛋白样蛋白(SjMLP)核酸疫苗.方法分子克隆常规操作将扩增产物SjMLP编码区基因序列克隆至载体pUCm-T中,然后亚克隆到真核表达载体pcDNA3.1(+)中.结果经酶切、PCR及测序鉴定表明所构建的质粒pUCm-T/SjMLP和pcDNA3.1(+)/SjMLP中含有所扩增的基因序列.结论成功地构建了含目的基因的真核表达质粒pcDNA3.1(+)/SjMLP,从而,为进一步对其进行DNA免疫系列研究工作奠定了基础.

Objective To clone the part gene coding for schistosoma japonicum mucin-like protein into the eukaryotic expression vector pcDNA3.1(+)and to construct a DNA vaccine.Methods TThe fragment of the SjMLP was amplified by PCR and cloned into cloning vector pUCm-T,then it was subcloned into eukaryotic expression vector pcDNA3.1(+).Results The coding region of SjMLP gene was verified after tested by PCR and by sequencing.Conclusion The eukaryotic expression plasmid was successfully constructed,which...