期刊论文

中文

实用预防医学

1006-3110

2019

26

2

129-133

10.3969/j.issn.1006-3110.2019.02.001

81572011:国家自然科学基金 81001318:国家自然科学基金 1038:湖南省大学生研究性学习和创新性实验计划

本校为第一机构

目的研究鹦鹉热衣原体（Chlamydia psittaci,Cps）巨噬细胞感染增强因子（microphage infectivity potentiator,Mip）在诱导人单核细胞THP-1产生前炎症细胞因子和对HeLa细胞凋亡的作用。方法用不同浓度的Cps Mip重组蛋白作用THP-1细胞,ELISA检测前炎症细胞因子IL-8和TNF-α的表达量;用荧光染色法和流式细胞术分析重组Cps Mip对HeLa细胞凋亡的作用。结果重组Cps Mip能以时间、剂量依赖方式诱导THP-1细胞分泌前炎症细胞因子IL-8和TNF-α;不同Cps Mip蛋白浓度实验组刺激THP-1细胞产生的前炎症细胞因子IL-8和TNF-α水平显著高于PBS对照组（P

Objective To study the role of microphage infectivity potentiator (Mip) of Chlamydia psittaci (C. psittaci) on the production of proinflammatory cytokines in human acute monocytic leukemia cell line (THP-1) cells and apoptosis inhibition in HeLa cells. Methods Different concentration of recombinant Mip protein was used to stimulate THP-1 cells, and the expression of interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) was detected by enzyme-linked immunosorbent assay (ELISA). The number of apoptosis cells was tested by fluorescence staini...