Objective To develop a novel method for the determination of hippuric acid and methyl hippuric acid in urine by ultra high performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Methods We employed reversed phase T3 column and acetonitrile-methanol-water (with 0.1% formic acid) as elution solvent, and then detected hippuric acid and methyl hippuric acid in urine by electrospray ionization mass spectrometry. Results This method successfully realized the baseline separation and detection of hippuric acid and three isomers of methyl hip...