期刊论文

Dichlorvos-induced oxidative damage in human neuroblastoma SK-N-SH cells

中文

实用预防医学

1006-3110

2017

24

6

646-649

10.3969/j.issn.1006-3110.2017.06.002

81172712:国家自然科学基金 81673227:国家自然科学基金

本校为第一机构

目的 研究敌敌畏(O,O-dimethyl-O-2,2-dichlorovinylphosphate,DDVP)对入神经母细胞瘤细胞(SK-N-SH)的氧化损伤作用. 方法 MEM培养基培养SK-N-SH细胞,细胞计数测定SK-N-SH细胞群体倍增时间;不同浓度(0～120 μmol/L) DDVP作用SK细胞48 h后,MTT法测定SK-N-SH细胞48h半数抑制浓度(IC50);巴氏染色后观察细胞形态变化;同时分别检测细胞内超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量的变化. 结果 SK-N-SH细胞群体倍增时间(36.28±1.46)h;48 h半数抑制浓度(IC50)为(105.22±5.80) μmol/L;随着DDVP染毒浓度的增高,细胞内MDA的含量逐渐增高,SOD、CAT活性逐渐降低,呈现明显的剂量一...

Objective To study the oxidative damage of O,O-dimethyl-O-2,2-dichlorovinylphosphate （DDVP） to human neuroblastoma SK-N-SH cells. Methods SK-N-SH cells were cultured by minimum essential medium, and counted to deter- mine the population doubling time. The SK-N-SH cells were treated with DDVP at different concentrations （0-120μmol/L） for 48 hours, and then the half maximal inhibitory concentration （ IC50 ） was measured by MTT assay. Cell morphological changes were observed under light microscopy by Giemsa staining; meanwhile, the activity...