期刊论文

中文

实用预防医学

1006-3110

2014

21

4

416-419

10.3969/j.issn.1006-3110.2014.04.010

本校为第一机构

目的 探讨双酚A对MCF-7细胞内整体DNA甲基化水平及甲基化转移酶的影响. 方法 分别将浓度为10-6 mol/L、10-7 mol/L的双酚A作用于MCF-7细胞72h后,免疫荧光法测5-甲基胞嘧啶的含量,RT-PCR法测DNMT1、DNMT3A、DNMT3B mRNA的表达. 结果 双酚A（10-6 mol/L、10-7 mol/L）作用72 h后,与溶剂对照组（0.1％DMSO）相比,5-甲基胞嘧啶的含量下降,差异有统计学意义（P＜0.05）;RT-PCR结果表明浓度为10-6 mol/L双酚A处理组的DNMT1的mRNA的表达下降,10-6 mol/L、10-7 mol/L双酚A组的DNMT3A和DNMT3B的mRNA的表达均增加,差异有统计学意义（P＜0.05）. 结论 双酚A能够引起MCF-7细胞整体DNA甲基化水平下调,其机制可...

Objective To investigate the effect of bisphenol A （BPA） on the general level of DNA methylation and the expression of DNA methyltransferases （DNMTs） in MCF-7 cells.Methods MCF-7 cells were respectively treated with 10-6 mol/L and 10-7 mol/L BPA for 72 hours.The DNA methylation levels were determined by 5-methylcytosine immunoflurescence assay.The mRNA expression of DNMT1,DNMT3A and DNMT3B were measured by RT-PCR.Results Compared with the control group （0.1% DMSO）,the amounts of 5-methylcytosine in the experimental groups decreased signif...