期刊论文

中文

实用预防医学

1006-3110

2011

18

10

1826-1829

10.3969/j.issn.1006-3110.2011.10.004

衡阳市社会发展科技支撑计划项目（2009ks31）；

本校为第一机构

目的 探讨丙烯酰胺(AA)对PC12细胞的细胞毒性及可能机制.方法 分别以0、62.5、125、250、500、1 000μmol/L AA为染毒剂量,染毒12 h后,采用MTT比色法检测丙烯酰胺对体外培养PC12细胞的增殖抑制作用,并用碱性单细胞凝胶电泳技术(single cell gel electrophoresis,SCGE)检测PC12细胞DNA的损伤作用、用MDA和T-AOC试剂盒检测细胞MDA和T-AOC改变.结果 染毒12 h后,细胞生长受到明显的抑制,且染毒剂量越高,抑制作用越明显(P＜0.01)；单细胞凝胶电泳实验表明,AA对体外培养PC12细胞的DNA有明显的损伤作用,细胞上清液中MDA含量随着染毒量的上升而升高,T-AOC含量随染毒量的上升而降低.结论 AA能够显著抑制P...

Objective To explore the cytotoxicity of acrylamide（AA） to PC12 cells and the possible mechanism. Methods Cells were exposed to 0,62.5,125,250,500,and 1,000 μmol/L AA,respectively,and after 12 hrs,the MTT method was used to detect the rate of cell proliferation and inhibition,alkaline single-celled gel electrophoresis technology was harnessed to obtain the result of DNA damage level of PC12 cells,and the MDA and T-AOC kit were applied to determine the content change of MDA and T-AOC in cell cultures. Results After 12 hrs AA administration,th...