期刊论文

中文

实用预防医学

1006-3110

2008

15

1

22-24

10.3969/j.issn.1006-3110.2008.01.006

湖南省教育厅资助项目（No.02C391）；

本校为第一机构

目的 研究GFP-ICP0融合蛋白瞬时高表达对活化巨噬细胞分泌活性的影响. 方法 构建GFP-ICP0融合蛋白真核细胞表达载体pEGFP/ICP0.将其转染巨噬细胞,用荧光显微镜观察GFP-ICP0融合蛋白在巨噬细胞内的表达与定位,并利用Western blot检测表达产物.通过GFP-ICP0融合蛋白在巨噬细胞内的瞬时高表达,在LPS诱导激活巨噬细胞后12、24、48 h时,测定活化巨噬细胞分泌TNF-α和IL-1β的含量. 结果 GFP-ICP0融合蛋白在巨噬细胞内瞬时高表达且定位于细胞核.GFP-ICP0融合蛋白在巨噬细胞内的高表达,使LPS诱导活化的巨噬细胞分泌TNF-α和IL-1β量明显增加(在12、24与48 h时与对照组差异有显著性,P＜0.01). 结论 ICP0...

Objective To detect the effect of over - expression of GFP - ICP0 fusion protein on secretion of TNF - α and IL - 1β in activated macrophages. Methods The eukaryotic expression vector of GFP - ICP0 fusion protein was constructed by ICP0 fragments subcloned to pEGFP - C1 24 hrs after transfection of pEGFP/ ICP0 into macrophages. Expression product of GFP- ICP0 fusion protein was analyzed by Western blot and observed localization under fluorescent microscope. The macrophage culture media were replaced with a medium containing LPS. During furthe...