期刊论文

Damage of Human Umbilical Vein Endothelial Cells Induced by Ethanol and Whether Its Injuring Mechanism is Related to the Signal Pathway of ADMA/NOS/NO

中文

实用预防医学

1006-3110

2008

15

2

293-297

10.3969/j.issn.1006-3110.2008.02.001

30572191:国家自然科学基金 B200508:湖南省卫生厅科学研究项目 DS02224:南华大学科研基金 5-2005-XQD-001:博士启动基金

本校为第一机构

目的 探讨乙醇诱导人脐静脉内皮细胞损伤效应及ADMA/NOS/NO信号机制.方法 用不同浓度的乙醇与人脐静脉内皮细胞共培养,观察细胞形态和活性,通过检测细胞上清液丙二醛(MDA)、非对称性二甲基精氨酸(AD-MA)、一氧化氮(NO)的含量和一氧化氮合酶(NOS)的活性等指标,观察乙醇对人脐静脉内皮细胞损伤的量-效关系(乙醇处理细胞24 h)和时-效关系(终浓度为100 mmol/L的乙醇分别于处理0、3、6、12、24、48 h后观察检测指标).实验分为正常对照组(加入与处理组等体积的D-Hank's液,乙醇浓度为0 mmol/L)、乙醇处理组(终浓度分别为25、50、100、200mmol/L的乙醇)、乙醇&VitC组(乙醇终浓度为100 mmol/L,VitC终浓度...

Objective To study the damage of human umbilical vein endothelial cells induced by ethanol and whether its injuring mechanism is related to the signal pathway of ADMA/NOS/NO. Methods HUVEOs - 12 was cultured in DMEM containing 10 % FOS. At 80% confluence, cells were washed with D Hank＇s fluid and cultured in DMEM containing 1% FOS for 24h. Cells were incubated in the presence or absence （control） of ethanol. To test the dose effect of ethanol- induced endothelial cells damage, the cells were treated with ethanol at the doses of 2.5, .50, 100...