期刊论文

Expression of Human IL- 15 Gene in CHO Cells and its Inhibitory Effect on Proliferation of Nasopharyngeal Carcinoma CellLine in Vitro

中文

实用预防医学

1006-3110

2007

14

3

653-655

10.3969/j.issn.1006-3110.2007.03.016

本校为第一机构

目的 在CHO细胞中进行人白细胞介素-15基因真核表达载体的稳定表达,并检测重组表达蛋白rhIL15对鼻咽癌细胞体外增殖的抑制作用.方法 将真核表达载体pcDNA3.1(+)-hIL-15转染CHO细胞,G418筛选稳定表达株;采用RT PCR和ELISA法对hIL 15的表达进行检测,MTT法检测rhIL-15对人鼻咽癌细胞系CNE-2Z体外增殖的抑制效应.结果 人IL-15在CHO细胞中获得稳定表达,RT-PCR显示细胞的rhIL-15 mRNA呈现高水平,ELISA检测到rhIL-15在细胞中的分泌性表达,浓度达(3.08±0.23)μg/ml,且所表达的人IL-15具有较强的抑制CNE-2Z鼻咽癌细胞增殖的作用.结论 成功地在CHO细胞中获得了hIL-15蛋白的高效、稳定表达,重组表达蛋白rh...

Objective The eukaryotic expression vector of hlL- 15 cDNA was expressed in Chinese hamster ovary （CHO） cells, and the inhibition effects of recombinant hlL- 15 protein on Nasopharyngeal carcinoma cell line proliferation in vitro were detected. Methods The recombinant expression plasmid pcDNA3.1 （ ＋ ） - hlL - 15 was transfected into CHO cells and the positive clone was screened by G418. Then the expression of hlL - 15 in CHO cells was detected by RT- PCR and ELISA. The inhibitory effects of recombinant protein hlL- 15 on proliferation of h...