期刊论文

Effect of Alcohol on Serum NOS Activity and ADMA Concentration in Rat Models

中文

实用预防医学

1006-3110

2007

14

2

315-318

10.3969/j.issn.1006-3110.2007.02.020

湖南省卫生厅科研课题资助（B200508） 致谢：感谢中南大学药学院湖南省心血管重点实验室全体同仁的指导和帮助.

本校为第一机构

目的研究不同浓度的酒精对大鼠血清中NOS活性及其内源性抑制物ADMA含量的影响。方法动物实验选用SD大鼠（雄性、56只、体重约250 g）灌胃给予不同剂量饮用酒处理组（红星二锅头,1 ml/100 g）,每天一次,连续2个月,建立酒精摄入的动物实验模型。实验分为7组（n=8）。（1）对照组,（2）低、中、高三个剂量的饮用酒组（红星二锅头,14 V/V2、8 V/V5、6 V/V）,（3）高脂饮食组,（4）高脂饮食联合中剂量饮用酒组（28 V/V）:灌胃给予红星二锅头的同时,给予高脂饲料,（5）维生素E保护组,在（4）组基础上,每天给予维生素E（100 mg/kg,灌酒前1 h灌胃给予）。2个月后,按常规取动物血清,测定MDA、NO的含量与N...

Objective To study the effect of alcohol on serum ADMA level and NOS activities in rat models. Methods Sprague Dawley male rats weighing about 250g were actively given with three doses of alcohol （14V/V, 28V/V, and 56V/V） by intragastric feeding .at a rate of 1mL/100g, one time per day for two months. On the 60th day, blood samples were collected from femoral arteries, centrifuged （3500rpm, 10min） and separated immediately. Serum levels of MDA （thiobarbituricacid method）, NO （Griess method） and ADMA （HPLO） were determined. Serum activ...