期刊论文

中文

实用预防医学

1006-3110

2005

12

2

219-222

10.3969/j.issn.1006-3110.2005.02.002

湖南省教育厅 2 0 0 2年课题资助 （NO .0 2C391 ）；

本校为第一机构

目的利用真核表达载体pEGFP/hDaxx在HeLa细胞中高表达hDaxx,研究hDaxx对HeLa细胞凋亡的影响.方法将pEGFP/hDaxx转染HeLa细胞,Western-blotting鉴定GFP-hDaxx融合蛋白的表达,荧光显微镜观察GFP-hDaxx融合蛋白在细胞内的表达及定位,用地塞米松诱导HeLa细胞凋亡,细胞用Giemsa染色后,观察细胞形态变化并计算HeLa细胞凋亡率.结果GFP-hDaxx融合蛋白在HeLa细胞中成功表达并定位于细胞核.地塞米松对照组与pEGFP对照组凋亡率无显著性差异(P＞0.05),而pEGFP/hDaxx组凋亡率显著降低(P＜0.01),即hDaxx即时高表达降低地塞米松诱导的HeLa细胞凋亡率.结论Daxx为调控蛋白,GFP-hDaxx融合蛋白在HeLa细胞中表达并定...

Objective To study the effects from overexpression of hDaxx on Dexamethasone-induced apoptosis in HeLa cells. Methods An eukaryotic expression vector pEGFP/hDaxx was used to transfect HeLa cells for overexpressing hDaxx. HeLa cells were respectively transfected with pEGFP/hDaxx and pEGFP, and the expression was identified by Western-blot. Furtherly, under fluorescent microscope the expression and localization of GFP-hDaxx fusion protein in HeLa cells was observed. Then, dexamethasone was added to HeLa cells medium for inducing apoptosis, the apoptosis rate of HeLa cells in morphology was count...