期刊论文

The Constructions of Recombinant Eukaryotic Expression Plasmids Including Both HBV S Gene and HCV C Gene

中文

实用预防医学

1006-3110

2003

10

2

146-148

10.3969/j.issn.1006-3110.2003.02.008

本校为第一机构

目的构建含HBV S基因及HCV C基因的嵌合基因真核表达质粒,为HBV和HCV嵌合基因疫苗研究奠定基础. 方法PCR扩增分别获得HBV preS1+preS2+S、preS2+S、S基因片段及HCV C基因片段,以pcDNA3.1(+)为载体,构建同时含HBV preS1+preS2+S、preS2+S或S基因与HCV C区基因的嵌合真核表达质粒S1S2SCpcDNA3.1、S2SCpcDNA3.1、SCpcDNA3.1.结果经酶切鉴定及测序分析,所构建的嵌合表达质粒均连接正确,与预计一致.结论嵌合基因真核表达质粒的成功构建,将为观察嵌合基因免疫及HBV、HCV基因疫苗的研究提供实验基础.

Objective To construct recombinant eukaryotic expression plasmids including both HBV S gene and HCV C gene, and to develop chimeric genetic vaccine of HBV and HCV. Methods Four gene fragments of HBV preS1+preS2+S, preS2+S, S and HCV C were amplified by PCR and these fragments were cloned into eukaryotic expression vector pcDNA3.1(+). Three recombinant eukaryotic expression plasmids were constructed and were named S1S2SCpcDNA3.1,S2SCpcDNA3.1,SCpcDNA3.1. These plasmids include HBV S (preS1+preS2+S, preS2+S or S) gene and HCV C gene simultaneously. Result These recombinant expression plasmids wer...