期刊论文

High glucose inhibits the growth of HT22 cells by inducing cell senescence and apopotsis

中文

蛇志

1001-5639

2017

29

3

267-269

10.3969/j.issn.1001-5639.2017.03.003

本校为第一机构

目的 探讨高糖（High glucose,HG）对小鼠海马神经元细胞HT22细胞的生长抑制作用,分析其是否通过HG诱导细胞衰老以及凋亡而实现.方法 采用台盼蓝染色计数法检测细胞生长状况并绘制生长曲线,β-半乳糖苷酶（Senescence asso-ciated acidic-β-galactosidas,SA-β-Gal）染色法检测衰老的HT22细胞,Hoechst 33258染色法检测HT22细胞凋亡形态.结果（1）HG（13.5、27、40.5 mg/ml,48 h）能显著抑制HT22细胞生长（P〈0.05,P〈0.01）;（2）HG（13.5、27、40.5 mg/ml）处理48 h可明显诱导SA-β-Gal染色阳性率增加（P〈0.001）;（3）HG（13.5、27、40.5 mg/ml,48 h）能诱导HT22细胞发生凋亡（P〈0.001）,且...

Objective To investigate the inhibitory effect of high glucose(HG)on the growth of HT22 cells(a kind of mouse hippocampal neurons)and analyze whether this inhibitory role is mediated by HG-induced cell senescence and apoptosis.Methods HT22 cells growth was measured by trypan blue staining and the growth curves were drawn.The senescent HT22 cells were detected by SA-β-Gal(Senescence Associated acidic-β-Galactosidas)staining.The apoptotic HT22 cells were observed by Hoechst 33258 staining.Results(1)HG(13.5,27,40.5mg/ml,48h)could significantly i...