期刊论文

Cai, S.-C.

中文

第二军医大学学报

0258-879X

2015

36

7

802-804

10.3724/SP.J.1008.2015.00802

湖南省教育厅科学研究项目（14C0909）； 湖南医药学院科学研究项目（2014KY02）~~；

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糖尿病(diabetes mellitus,DM)特征性病理生理改变是高血糖、碳水化合物和脂肪代谢紊乱导致的胰岛素抵抗或胰岛素分泌的绝对或相对不足,以2型糖尿病(type 2 diabetes mellitus, T2DM)为主。T2DM发病原因主要是胰岛素信号转导通路破坏,产生胰岛素抵抗(insulin resistance, IR)。蛋白激酶B (protein kinase B, PKB)又称Akt,是胰岛素信号转导过程中的关键分子之一,磷酸化PKB (p-PKB)是其活性形式。

Objective: The objective of present study was to examine the changes of melatonin receptor agonist Neu-P11 on expression of PKB and P-PKB in 3T3-L1 adipocytes and to research the variation mechanisms.Methods: 3T3-L1 pre-adipocytes were cultivated and differentiated in adipogenic cocktail by IBMX, DEX and insulin; Confirm the establishment of insulin resistance model. The changes of PKB and P-PKB protein expressions before and after drug action were detected by Western blot; Results: Expression of PKB and P-PKB reduced significantly by compariso...