This study investigated the chromatographic characteristics of irradiated vitamins and their feasibility for dose measurement by using high performance liquid chromatography (HPLC). Water-soluble vitamins in the B family, including thiamine hydrochloride (TH), riboflavin (RF) and nicotinic acid (NA), were used for comparative study. The results showed that riboflavin was sensitive to irradiation, and the content changes of RF were linearly correlated with irradiation doses. Combining the analyses of ultraviolet and fluorescence spectra, the sui...
