期刊论文

miR-19b down- regulation expression of Kir2.1 protein in H9c2 rat cardiomyocytes

中文

微量元素与健康研究

1005-5320

2015

32

2

1-3+5

国家自然科学基金项目（30900625/H0205）；

本校为第一机构

目的:检测miR-19b对大鼠H9c2心肌细胞Kir2.1表达的影响,探讨miRNA参与房颤发生发展的机制。方法:培养大鼠H9c2心肌细胞,构建miR-19b过表达质粒、miR-19b干扰质粒、阴性序列质粒,通过慢病毒载体感染大鼠H9c2心肌细胞;倒置荧光显微镜观察感染效率,qRT-PCR检测H9c2细胞中miR-19b mRNA表达水平。qRT-PCR检测H9c2细胞中Kir2.1mRNA水平。Western blot检测H9c2细胞中Kir2.1蛋白表达水平。结果:miR-19b慢病毒载体成功感染H9c2细胞;qRT-PCR检测,与阴性序列组相比,miR-19b过表达组Kir2.1 mRNA表达水平降低,miR-19b干扰组Kir2.1 mRNA表达水平升高;Western blot检测,与阴性序列质粒组相比,miR-19b过表达组Ki...

Objective： To examine the effect of miR-19 b on expression of Kir2.1 protein in H9c2 rat cardiomyocytes,and investigate mechanism of miRNA involved in the development of atrial fibrillation.Methods： Culture H9c2 rat cardiomyocytes; MiR-19 b plasmid and anti- miR- 19 b plasmid was stably transfected to H9c2 cells by lentiviral vector system; Inverted fluorescence microscopy and qRT- PCR to detect the transfection efficiency; Kir2.1 mRNA level was detected by qRT- PCR,Kir2.1 protein expression levels was analyzed by western blot.Results： miR- ...