期刊论文

Intravaginal infection with Chlamydia muridarum can cause renal lesions as a result of the infection ascending through the genitourinary tract in the absence of IL-12

中文

中国病原生物学杂志

1673-5234

2019

14

1

6-11

10.13350/j.cjpb.190102

No.81572011:国家自然科学基金 31600150:国家自然科学基金 No.2016JJ3103:湖南省自然科学基金

本校为第一机构

目的了解在Il-12缺失小鼠中衣原体经外生殖道进入肾脏导致肾脏病变情况。方法试验用野生型(wild type,wt)、IL-12p35 KO及IL-12p40 KO小鼠,每组10只,经阴道分别接种1×10~4 IFUs鼠衣原体(MoPn),于感染后第45、87和100 d处死小鼠。分离其肾脏、尿道、膀胱、肺、肝、脾、心脏组织,分别加入预冷的SPG,经组织匀浆器匀浆、超声破碎后做倍比稀释。取稀释液感染Hela细胞,培养24 h后进行间接免疫荧光试验,计数各组织中衣原体包涵体数量。其余5只小鼠的组织进行HE染色,检测其病理变化。结果感染后第45、87和100 d,3种小鼠肝、肺、心脏和脾脏组织中均未检出活的衣原体;而在肾脏、尿道、膀胱组织中除wt小鼠...

Objective To explore the manner in which intravaginal infection with Chlamydia muridarum causes renal lesions in the absence of Il-12.Methods C57BL/6J wild-type (wt)and mice deficient in IL-12p35 (IL-12p35 KO)and IL-12p40 (IL-12p40 KO)were intravaginally inoculated with 1×10~4 IFUs of live C.muridarum.Ten mice in each group were sacrificed on day 45,87,and 100 after infection.Kidney,urinary tract,bladder,lung,liver,spleen,and heart tissues were isolated and added to pre-cooled SPG,homogenized using a tissue homogenizer,sonicated,diluted,and us...