期刊论文

中文

中国病原生物学杂志

1673-5234

2012

7

10

721-723,742

10.13350/j.cjpb.2012.10.011

国家自然科学基金项目（No.30973402/H1601）； 湖南省教育厅重点项目（No.11A102）； 湖南省“高校科技创新团队”项目（湘教通2010-212）；

本校为第一机构

目的将HPV16 E2与IL-12真核表达载体联合免疫小鼠,检测小鼠产生的特异性体液免疫和细胞免疫应答水平,为HPV16诱发的宫颈癌治疗性核酸疫苗的研制奠定实验基础。方法40只BALB/c小鼠随机分成PBS组、pcDNA3.1(+)空质粒组、pcDNA3.1(+)/IL-12组、pcDNA3.1(+)/HPV16 E2组以及pcDNA3.1(+)/HPV16 E2+pcDNA3.1(+)/IL-12联合组,每组8只,共免疫4次,每2周1次。于免疫前1 d及第8周采血,分离血清,-20 ℃保存;颈椎脱臼处死小鼠,无菌取脾脏,制备脾细胞悬液。ELISA法测定小鼠血清HPV16 E2 IgG抗体水平及脾细胞培养上清IFN-γ、IL-4水平;MTT比色法检测脾淋巴细胞的增殖。结果免疫8周后,血清IgG抗体A_(450)值E2组和...

Objective In order to provide an experimental basis for studying remedial vaccines to treat infection with human papillomavirus type 16（HPV16）,the immunological competence of BALB/c mice was analyzed after they were inoculated with the eukaryotic expression vectors HPV16 E2 and IL-12.Methods A total of 40 BALB/c mice was randomly divided into 5 groups,the PBS Group,pcDNA3.1（＋） Group,pcDNA3.1（＋）/IL-12 Group,pcDNA3.1（＋）/HPV16 E2 Group（the single DNA vaccine group）,and the pcDNA3.1（＋）/HPV16 E2＋pcDNA3.1（＋）/IL-12 Group（the combi...