期刊论文

PIK3CA expression in gastric cancer tissues and the correlation between PIK3CA and metastasis

中文

实用医学杂志

1006-5725

2011

7

1173-1175

10.3969/j.issn.1006-5725.2011.07.018

湖南省自然科学基金项目（编号:09JJ3060）； 湖南省高校科技平台基金（编号:09A072）； 广东省医学科研基金项目（编号:A2007284）；

本校为第一机构

目的:探讨PIK3CA在胃癌组织中的表达及其与转移的关系.方法:采用实时定量PCR和免疫组织化学方法检测PIK3CA基因在正常胃黏膜组织、原发胃癌组织、淋巴结转移及远处转移胃癌组织中mRNA和蛋白表达水平,并比较各组间的差别及其与临床病理特征的关系.结果:淋巴结转移胃癌组织中PIK3CA mRNA表达水平最高,分别约为正常胃黏膜组织及原发胃癌组织的5倍和2倍,差异具有统计学意义(P ＜ 0.05),但与远处转移胃癌组织比较差异无统计学意义.免疫组化结果表明,PIK3CA蛋白在原发胃癌组织、淋巴结转移及远处转移胃癌组织中阳性率分别为35%、67% 和61%.与原发胃癌组织比较,淋巴结转移及远处转移胃癌组织中PIK3CA蛋...

Objective To explore PIK3CA expressions in gastric cancer tissues and the correlation between PIK3CA expression and metastasis. Methods PIK3CA mRNA and protein levels of normal gastric mucosa, primary gastric cancer tissues, gastric cancer specimens in lymph node metastasis and distant metastasis were detected respectively by Real-time quantitative PCR and immunohistochemistry. In addition, the difference of PIK3CA expression in each group and their relationship with clinical pathological characteristics were analyzed. Results PIK3CA mRNA in ly...