期刊论文

中文

实用医学杂志

1006-5725

2010

26

5

726-728

10.3969/j.issn.1006-5725.2010.05.009

湖南省卫生厅研究基金资助项目（编号:B2006-098）；

本校为第一机构

目的：观察高密度脂蛋白（HDL）对THP-1巨噬细胞B类Ⅰ型清道夫受体（SR-BI）的表达及胆固醇流出的影响。方法：用HDL及氧化低密度脂蛋白（ox-LDL）处理THP-1细胞，采用RT-PCR、Western印迹及液体闪烁计数法观察细胞SR-BI的表达及细胞内胆固醇流出的变化。结果：ox-LDL下调THP-1巨噬细胞中SR-BI的表达，而HDL则上调THP-1巨噬细胞中SR-BI的表达：液体闪烁计数法检测发现OX—LDL减少细胞中胆固醇的流出，HDL可以增加细胞胆回醇的流出，且HDL的效应呈浓度依赖性。结论：HDL可能通过上调THP-1巨噬细胞SR-BI的表达．促进巨噬细胞内胆固醇流出。

Objective To investigate the influences of HDL on SR-BI expression and cholesterol efflux in THP-1 cell line. Methods THP-1 cells were treated with ox-LDL or HDL at different concentrations. [^3H]-labeled cholesterol flux was determined by liquid scintillation; expression levels of SR-BI mRNA and protein were determined by reverse transeription-polymerase chain reaction and Western blot, respectively. Results ox-LDL downregulated but HDL upregulated the expression of SR-BI in THP-1 cells in a dose-dependent manner. Ox-LDL decreased while HDL increased the efflux of intracellular cholesterol in...