期刊论文

Down-regulation of miR-34a-5p by ionizing radiation promotes radiation-induced lung epithelial-mesenchymal transition

中文

西安交通大学学报(医学版)

1671-8259

2020

41

3

333-339

10.7652/jdyxb202003005

No.81773359:国家自然科学基金 31470827:国家自然科学基金 31270894:国家自然科学基金

本校为第一机构

目的探究辐射下调miR-34a-5p在辐射诱导上皮间质转化(EMT)中的作用及其机制。方法Western blotting和流式细胞术检测~(60)Co γ射线照射后肺上皮细胞中EMT相关标志蛋白的表达变化;RT-qPCR检测~(60)Co γ射线照后不同时间点A549细胞和BEAS-2B细胞中miR-34a-5p的表达;将miR-34a-5p过表达或敲低后,Western blotting检测辐射诱导时EMT的表达。生物信息学预测miR-34a-5p的靶基因,RT-qPCR和Western blotting检测miR-34a-5p对靶基因的调控效果。结果6 Gy ~(60)Co γ射线照射后48 h肺上皮细胞发生EMT,RT-qPCR检测显示miR-34a-5p表达下调;过表达miR-34a-5p可逆转辐射诱导的EMT,下调表达miR-34a-5p时,CD44...

Objective To explore the role and mechanism of radiation-induced miR-34a-5P in radiation-induced epithelial-mesenchymal transition (EMT).Methods We used Western blotting and flow cytometry to detect EMT-related marker protein expression in lung epithelial cells after ~(60)Co γ-ray irradiation.The expression of miR-34a-5p in lung cancer cells A549 and normal lung cells BEAS-2B was initially screened by Real-time qPCR (RT-qPCR) at different time points after ~(60)Co γ-ray irradiation.The effect of miR-34a-5p on radiation-induced EMT was detecte...