A universal aptasensing platform based on cryonase-assisted signal amplification and graphene oxide induced quenching of the fluorescence of labeled nucleic acid probes: application to the detection of theophylline and ATP

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成果类型：

期刊论文

作者：

Lou, Yi-Fei;Peng, Yong-Bo;Luo, Xiaowei;Yang, Zhiming;Wang, Ruifeng;...

通讯作者：

Cui, Liang;Huang, Jiahao

作者机构：

[Yang, Zhiming; Li, Lingxiangyu; Lou, Yi-Fei; Wang, Ruifeng; Cui, Liang; Sun, Dewen] Zhejiang Sci Tech Univ, Dept Chem, Hangzhou 310008, Zhejiang, Peoples R China.

[Huang, Jiahao; Peng, Yong-Bo] Southern Med Univ, Sch Biomed Engn, Guangdong Prov Key Lab Construct & Detect Tissue, Guangzhou 510515, Guangdong, Peoples R China.

[Peng, Yong-Bo] Hunan Univ, Coll Life Sci, Mol Sci & Biomed Lab MBL, Changsha 410082, Hunan, Peoples R China.

[Peng, Yong-Bo] Univ Hong Kong, Li Ka Shing Fac Med, Sch Chinese Med, Estates Bldg,10 Sassoon Rd, Hong Kong 00852, Peoples R China.

[Luo, Xiaowei] Univ Washington, Dept Chem, Seattle, WA 98195 USA.

通讯机构：

[Cui, Liang] Z

[Huang, Jiahao] S

Zhejiang Sci Tech Univ, Dept Chem, Hangzhou 310008, Zhejiang, Peoples R China.

Southern Med Univ, Sch Biomed Engn, Guangdong Prov Key Lab Construct & Detect Tissue, Guangzhou 510515, Guangdong, Peoples R China.

语种：

英文

关键词：

Aptamer;ATP;Detection limit;Nucleic acid molecular probes;Theophylline;Signal amplification

期刊：

Microchimica Acta

ISSN：

0026-3672

年：

2019

卷：

186

期：

页码：

1-9

DOI：

10.1007/s00604-019-3596-1

基金类别：

Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY19B050009]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21705076, 81602206]; Hong Kong Scholars Program [XJ 2017054]

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本校为其他机构

院系归属：

电气工程学院

摘要：

This study describes a universal fluorometric method for sensitive detection of analytes by using aptamers. It is based on the use of graphene oxide (GO) and cryonase-assisted signal amplification. GO is a strong quencher of FAM-labeled nucleic acid probes, while cryonase digests all types of nucleic acid probes. This makes the platform widely applicable to analytes for which the corresponding aptamers are available. Theophylline and ATP were chosen as model analytes. In the absence of targets, dye-labeled aptamers are in a flexible single strand state and adsorb on the GO. As a result, the pr...

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A universal aptasensing platform based on cryonase-assisted signal amplification and graphene oxide induced quenching of the fluorescence of labeled nucleic acid probes: application to the detection of theophylline and ATP

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