期刊论文

Effects of propofol on TGF-β1 induced activation of hepatic stellate cells and its mechanism

中文

中国应用生理学杂志

1000-6834

2020

36

3

273-278

10.12047/j.cjap.5929.2020.060

湖南省自然科学基金省市联合基金项目（2017JJ4049）；

本校为第一机构

目的: 观察丙泊酚对转化生长因子-β1(TGF-β1)诱导的肝星状细胞系HSC2-T6细胞激活的影响并探讨其可能的作用机制。方法: 实验分为对照组、TGF-β1组、丙泊酚组、TGF-β1+丙泊酚组、雷帕霉素组、TGF-β1+丙泊酚+雷帕霉素组。先用含雷帕霉素(5 μmol/L)DMEM培养液培养1 h,用丙泊酚(100 μmol/L)处理1 h,然后再加入TGF-β1(5 ng/ml)继续共同培养24 h。细胞的增殖水平通过MTT法测量,细胞培养液上清中透明质酸(HA)、IV型胶原(IV-C)和层粘连蛋白(LN)的浓度采用ELISA法测量,细胞的超微结构采用透射电镜观测,α-平滑肌肌动蛋白(α-SMA)、哺乳动物雷帕霉素靶蛋白(mTOR)、磷酸化mTOR(p-mTOR)及自噬相关基...

Objective:To observe the effects of propofol on the activation of hepatic stellate cell line HSC2-T6 induced by transforming growth factor-beta 1(TGF-β1)and explore its possible mechanism.Methods:The cells were divided into control group,TGF- β1 group,propofol group,TGF-β1 + propofol group,rapamycin group,TGF-β1 + propofol + rapamycin group.Cells were treated with rapamycin(5 μmol/L)for 1 hour,propofol(100 μmol/L)for 1 hour,then TGF-β1(5 ng/ml)was added to co-culture for 24 hours.Cell proliferation was measured by MTT assay.The concentra...