期刊论文

Effect of arecoline on proliferation and apoptosis of MCF-7 human breast cancer cells

中文

中国应用生理学杂志

1000-6834

2016

32

4

370-372

10.13459/j.cnki.cjap.2016.04.021

教育部留学归国基金（教外司留[2014]1685号） 中国博土后科学基金（2012M511384） 湖南省科技厅（2015JC3085）项目资助 南华大学留学归国基金项目（2013XQD49）

本校为第一机构

目的:观察槟榔碱对人乳腺癌细胞(MCF-7)增殖和凋亡的影响,并探讨其机制。方法:采用四甲基偶氮唑盐(MTT)法检测不同浓度(0、10、30、50、100、300、500 μmol/L)槟榔碱对MCF-7细胞增殖的影响,Hoechst 33342染色和流式细胞术检测细胞凋亡,Western blot法检测Bax,Bcl-2和P53蛋白表达。结果:低浓度(0、10、30、50 μmol/L)槟榔碱不影响细胞的增殖和凋亡;而高浓度(100、300、500 μmol/L)槟榔碱呈浓度依赖性抑制MCF-7细胞增殖、诱导MCF-7细胞凋亡、提高P53和Bax蛋白表达、降低Bcl-2蛋白表达。结论:高浓度槟榔碱抑制MCF-7细胞增殖、诱导凋亡,其机制可能与提高P53和Bax蛋白表达,降低Bcl-2蛋白表达有关。...

OBJECTIVE: To observe the effects of arecoline on proliferation and apoptosis of MCF-7 human breast cancer cells and to ex-plore its possible mechanism. METHODS: Human breast cancer MCF-7 cells were treated with arecoline at the concentrations of 0,10,30,50, 100,300,500mumol/L, the cell proliferation were detected by MTT assay, cell apoptosis were analyzed by Hoechst 33342 staining and flow cy-tometry, the protein expression of Bax,Bcl-2 and P53 were detected by Western blot. RESULTS: Low concentration(0,10,30, 50 mumol/L) arecoline had no effect on the proliferation and apoptosis of MCF-7. Ho...