期刊论文

Ling, H.Y.

中文

中国应用生理学杂志

1000-6834

2011

27

4

391-395

10.13459/j.cnki.cjap.2011.04.013

30971170:国家自然科学基金 81000328:国家自然科学基金

本校为第一机构

目的：探讨小鼠间充质干细胞（MSCs）定向诱导分化成脂肪细胞微小RNA（miRNA）表达的变化，为进一步研究miRNA调控MSCs向脂肪细胞分化的分子机制奠定基础。方法：采用全骨髓体外分离结合差速贴壁法纯化扩增C57BL／6小鼠MSCs，形态学观察细胞生长情况，并用免疫组化方法鉴定细胞表面抗原CD29、CIM4和CD34的表达。脂肪细胞分化诱导剂诱导MSCs分化为脂肪细胞，利用油红O染色，判断MSCs成脂分化情况。运用rrfiRNA芯片技术检测MSC8和脂肪细胞中差异表达的miRNA。结果：①倒置显微镜下观察，传5代后可获得均一性较高的MSCs；免疫组化显示90％以上的骨髓间质干细胞CD29、CD44阳性，CD34阴性。MSCs经脂肪...

OBJECTIVE: To explore miRNA expression change of differentiation of mice marrow mesenchymal stem cells (MSCs) into adipocytes, which lay the foundation for further studies on molecular mechanism of miRNA regulating the differentiation of MSCs into adipocytes. METHODS: C57BL/6 mice MSCs were isolated, cultured through the whole bone marrow method, amplified by the differential adherent method. Cell growth was observed by morphology and the expression of superficial antigen CD29, CD44, CD34 were detected through immunohistochemistry. MSCs was induced to differentiation into adipocytes with adipo...