期刊论文

Effects of leptin on disposition of triglyceride in L-02 fatty liver cells

中文

实用药物与临床

1673-0070

2011

14

5

373-375

10.3969/j.issn.1673-0070.2011.05.004

本校为第一机构

目的 观察瘦素对人L-02脂肪变肝细胞甘油三酯合成的影响,探讨瘦素在非酒精性脂肪性肝病发生、发展中的作用.方法 人肝L-02细胞在50%胎牛血清1640培养基中造模24 h.设立正常组、模型组、处理组,处理组分为3个亚组,分别应用瘦素10-7 mol/L、10-6 mol/L、10-5 mol/L处理24 h.均行油红"O"染色,观察脂肪化情况,RT-PCR测CA羧化酶mRNA表达.结果 油红"O"染色显示,50%胎牛血清的1640培养基24 h能成功制造肝细胞脂肪变性模型;加入瘦素处理后,脂肪化程度减轻,且与瘦素呈剂量依赖性关系;RT-PCR显示,肝细胞脂肪变性后,乙酰辅酶A羧化酶mRNA表达增强,加入瘦素处理后,乙酰辅酶A羧化酶mRNA表达明显减弱,与瘦素呈剂...

Objective To explore the effect of leptin to triglyceride＇s disposition in normal and nonalcoholic induced fatty liver cell.Methods The fatty liver cell model w as made by L-02 liver cells w ith 50% inactivation embryo blood serum for 24 h.The cells w ere randomized into normal group,model group,treatment 1 group（10-7 mol / L leptin）,treatment 2 group（10-6 mol / L leptin）,treatment 3 group（10-5 mol / L lepton）,the treatment group w as treated for 24 h.The fatty degeneration of liver w as observed by oil red staining,and RT-PCR w as used ...