期刊论文

Effect of Low-doseαParticle Irradiation on Primitive Hematopoietic Molecular Marker in Zebrafish Embryo

Ding, Dexin(dingdxzzz@163.com)

中文

原子能科学技术

1000-6931

2021

55

4

761-768

10.7538/yzk.2020.youxian.0300

国防基础科研项目资助（JCKY2016403C001）； 湖南省自然科学基金面上项目资助（2020JJ4523）；

本校为第一机构

为探究低剂量α粒子辐照对斑马鱼初级造血的影响,使用241 Am作为α粒子辐照源,对脱绒毛膜后的5 hpf胚胎进行0、0.425、0.85、1.7 mGy的辐照,采用整体原位杂交技术检测了约14 hpf斑马鱼胚胎初级造血分子标记物gata1a和spi1b的表达,采用实时荧光定量PCR技术检测了22 hpf斑马鱼胚胎初级造血分子标记物gata1a和spi1b的相对表达,并采用联大茴香胺染色法检测了48 hpf血红蛋白含量的变化.通过与对照组相比发现,0.425 mGy剂量组的gata1a表达无显著性差异(p>0.05),spi1b表达略有减少(p

In order to investigate the effect of low-doseαparticle irradiation on primitive hematopoiesis in zebrafish embryos,the 5hpost fertilization (hpf)dechorionated zebrafish embryos were irradiated at doses of 0,0.425,0.85and 1.7mGy,respectively, by using 241 Am as theαparticle irradiation source,the expressions of primitive hematopoietic markers gata1aand spi1bin the about 14hpf zebrafish embryos were detected by the whole-in-situ hybridization(WISH),and that in the 22hpf zebrafish embryos were analyzed by the real-time fluorescence quantitative...