期刊论文

Effect of γ-ray Irradiation on Developmental Morphology of Liver, Spleen and Pronephros in Zebrafish Embryo

Ding, De-Xin(dingdxzzz@163.com)

中文

原子能科学技术

1000-6931

2017

51

10

1886-1892

10.7538/yzk.2017.youxian.0014

国防基础重点基金资助项目（B3720132001）； 国家自然科学基金重点项目资助（U1401231）； 国防基础科研计划资助项目（JCKY2016403C001）；

本校为第一且通讯机构

为研究γ电离辐射对水生生物重要器官发育形态学的影响,以斑马鱼(Danio rerio)作为模式生物,研究了胚胎发育早期(受精后5 h,5 hpf)接受急性γ辐照(累积剂量为0.25、0.5、1 Gy,剂量率为47.79 cGy/min)后,受精后3 d(3 dpf)的幼鱼卵黄囊面积以及3 dpf和5 dpf的幼鱼肝、肾和脾发育形态学的变化。结果显示:3 dpf的幼鱼卵黄囊面积随剂量的增大而增大;3 dpf的幼鱼肝脏中空泡增加、肝脏局部出现空洞;5 dpf的幼鱼肝脏尺寸缩减、肝细胞内空泡增加且细胞核变形、血窦较小以及有核红细胞变形等;但前肾和脾的发育形态并没有出现异常。随后对3 dpf的幼鱼肝细胞超微结构进行观察,发现γ辐照使幼鱼肝脏细胞线粒体...

Zebrafish was used as a model organism to study the effects of γ-ray irradiation on the developmental morphology of important organs in the aquatic organisms. Different doses of gamma rays (0.25, 0.5, 1 Gy) at the dose rate of 47.79 cGy/min were used to irradiate zebrafish embryos at early development stage (5 hours post-fertilization, 5 hpf). The changes of yolk sac area at 3 days post-fertilization (3 dpf) and the changes of the morphology of liver, spleen and pronephros at 3 dpf and 5 dpf after γ-ray irradiation were analyzed, respectively. It is found that the area of yolk sac ...