期刊论文

Improvement of CGRP on vascular endothelial cells insulin resistance via activation of cAMP /PPARγ/eNOS pathway

中文

中国临床药理学与治疗学

1009-2501

2019

24

6

615-622

10.12092/j.issn.1009-2501.2019.06.003

国家自然科学基金项目(81173060) 湖南省教育厅科学研究项目(17C1400) 湖南省研究生科研创新项目(CX2017B544).

本校为第一机构

目的:探讨降钙素基因相关肽(CGRP)对人脐静脉内皮细胞胰岛素抵抗的保护作用及其机制。方法:用人脐静脉内皮细胞(HUVEC)建立胰岛素抵抗细胞模型(irHUVEC),分别用葡萄糖氧化酶法和蒽酮-硫酸法检测细胞葡萄糖消耗能力和糖原合成能力; RT-PCR和Western blot分别检测目的蛋白和基因表达。结果: 33.3 mmol/L的高糖和5 μmol/L的胰岛素处理24 h可以成功诱导内皮细胞产生胰岛素抵抗。与正常HUVEC相比,irHUVEC体积变大,边界模糊,形态异常;当细胞用高糖和高胰岛素分别诱导24 h和48 h时, irHUVEC的葡萄糖消耗量分别减少20%和31%,并且糖原合成分别减少了55%和64%,差异都具有显著性;同时,过氧化物酶增殖体激活...

AIM : To explore the protective effect and the mechanism of calcitonin gene-relatedpeptide (CGRP) on the insulin-resisted human umbilical vein endothelial cells (irHUVECs). METHODS : The irHUVECs was established using human umbilical veinendothelial cells (HUVECs), gucose oxidase (GOP-POD) method and anthrone-sulfuric acid method were employed to detect glucose consumption ability and glycogen synthesis ability, respectively. RT-PCR and Western blot were used to detect the mRNA and protein expressions of peroxisome proliferator-activated recept...