期刊论文

Liao, M.-C.

中文

肿瘤

1000-7431

2012

32

12

969-973

10.3781/j.issn.1000-7431.2012.12.004

本校为第一且通讯机构

目的:研究咖啡酸(caffeic acid)与化疗药物顺铂(cisplatin, DDP)联合应用对人肺腺癌A549细胞增殖及凋亡的影响,并初步探讨其可能的分子作用机制。方法:体外培养A549细胞,采用MTT法检测咖啡酸与DDP单药或联合用药对A549细胞增殖抑制率的影响;Hoechst33258染色观察细胞凋亡形态学的变化,FCM检测细胞凋亡率;蛋白质印迹法检测凋亡相关蛋白Bcl-2、Bax及caspase-3表达水平的改变。结果:咖啡酸或DDP单药或联合用药均对A549细胞有抑制细胞增殖和诱导细胞凋亡的作用,且两药联合应用具有协同作用。蛋白质印迹法检测结果表明,咖啡酸与DDP联合用药可协同抑制Bcl-2蛋白的表达,增强Bax的表,活化caspase-3蛋白的...

Objective: To investigate the growth inhibition effect of caffeic acid and DDP (cisplatin) on human lung adenocarcinoma A549 cells in vitro, and to explore the possible mechanism. Methods: A549 cells were cultured in vitro. The effect of caffeic acid alone or in combination with DDP on proliferation of A549 cells was detected by MTT assay. The effect of caffeic acid alone or in combination with DDP on apoptosis of A549 cells was detected by Hoechst33258 immunofluorescence staining and flow cytometry. The expressions of Bcl-2, Bax and caspase-3 ...