期刊论文

Duan, X.-M.

中文

肿瘤

1000-7431

2008

28

3

224-227

10.3781/j.issn.1000-7431.2008.03.011

湖南省卫生厅科研基金资助项目（编号:B2005-178）；

本校为第一机构

目的：研究羟基磷灰石（hydroxyapatite，HA）纳米颗粒载体携带人粒-巨噬细胞集落刺激因子（human granulocyte macrophage colony stimulating factor，hGM-CSF）基因转染HepG2细胞，以及其对细胞生长的影响，为基因修饰的肝癌肿瘤疫苗的临床研究奠定基础。方法：采用MTF法检测HA纳米颗粒载体对HepG2细胞生长的影响；采用HA纳米颗粒载体转染技术。将携带有hGM-CSF基因的表达质粒导入HepG2细胞中，G418筛选抗性细胞，限制性稀释法挑选单克隆；RT-PCR法鉴定hGM-CSF的整合和表达，ELISA法测定细胞培养液中的hGM-CSF分泌量和持续的时间；FCM法分析hGM-CSF基因对HepG2细胞生长的影响。结果：MTT结果...

Objective： To observe the impact of transfection of human granulocyte macrophage colony stimulating factor （ hGM- CSF） mediated by hydroxyapatite（HA） nanoparticles on the growth of HepG2 cells in order to lay the foundation for further studying gene-modified tumor vaccines of hepatoma in clinic. Methods：The proliferation of HepG2 cells was measured by MTT assay. The plas- mids containing hGM-CSF gene were transfected into HepG2 cells mediated by HA nanoparticles. Subsequently, resistant cells were selected by G418 screening. The monoclona...