期刊论文

Effects of K-ras gene silence on the expression of oncogenes in HBE cells treated with PM_(2.5)

中文

中华劳动卫生职业病杂志

1001-9391

2021

39

11

825-830

10.3760/cma.j.cn121094-20210107-00007

深圳市科技研发基础研究项目(JCYJ20190807102205480) 深圳市医疗卫生三名工程(SZSM201611068) 深圳市医学重点学科建设经费(SZXK067)。

本校为第一机构

目的探讨K-ras基因对PM_(2.5)染毒人支气管上皮(HBE)细胞部分癌基因和抑癌基因表达的影响。方法于2019年9月,根据K-ras基因mRNA序列,设计合成干扰序列,转染HBE细胞构建K-ras基因沉默细胞。用10、50 µg/ml PM_(2.5)混悬液及10 µmol/L Cr~(6+)分别染毒HBE细胞和K-ras基因沉默细胞,实时荧光定量PCR检测c-myc 、c-fos、N-ras、cyclin-D1、p16、p53基因的mRNA表达水平,Western blot检测c-myc和p53蛋白表达水平。结果K-ras基因沉默细胞中K-ras基因mRNA表达水平下降(80.5%± 3.6%),K-ras蛋白表达水平下降(58.9%±4.7%)(P

Objective To explore the effects of K-ras gene on the expressions of oncogenes and cancer suppressor genes in human bronchial epithelial (HBE) cells which were exposed to PM_(2.5). Methods According to the mRNA sequence of K-ras gene provided by GenBank in September 2019, interference sequences were designed and synthesized,and the recombinant lentiviral vector was transfected into HBE cell to construct the K-ras gene-silenced cells. HBE cells and K-ras gene-silenced cells were exposed to 10 µg/ml, 50 µg/ml PM_(2.5) suspension and 10 µmol/L ...