期刊论文

Effect of c-myc gene silence on the expression of oncogenes and apoptotic genes in hepatocytes treated with PM_(2.5)

中文

中华劳动卫生职业病杂志

1001-9391

2020

38

9

657-663

10.3760/cma.j.cn121094-20200326-00056

深圳市科技研发基础研究项目（JCYJ20170413101713324）；

本校为其他机构

目的:构建c-myc基因沉默肝细胞株，研究c-myc基因沉默对PM2.5染毒L02肝细胞凋亡基因和癌基因表达的影响。方法:根据GenBank提供的c-myc基因mRNA序列，设计合成3条干扰序列，将重组慢病毒载体转染L02肝细胞。利用实时荧光定量PCR（Q-PCR）和蛋白免疫印迹法（Western blotting）对c-myc基因沉默效果进行鉴定。用L02肝细胞、c-myc基因沉默细胞作为试验对象，用浓度为50 μg/ml的PM 2.5水溶物和10 μmol/L阳性对照Cr 6+染毒24 h即PM 2.5组、Cr 6+组，同时设立空白对照组；荧光定量PCR检测癌基因（c-myc、c-fos、k-ras、p53）和凋亡基因[半胱氨酸蛋白酶（Caspase）-3、Caspase-8、Caspase-9]的mR...

Objective:To construct the c-myc gene silenced hepatocytes, study the effect of c-myc gene silence on expression of oncogenes and apoptosis genes in hepatocytes treated with PM2.5.Methods:According to the c-myc gene mRNA sequence provided by GenBank, three interfering sequences were designed and synthesized, the recombinant lentiviral vector was transfected into L02 hepatocytes. The real-time quantitative PCR and western blotting were used to identify the effect of c-myc gene silencing. L02 cells and c-myc gene silenced cells were used as exper...