期刊论文

中文

中华劳动卫生职业病杂志

1001-9391

2018

36

5

321-326

10.3760/cma.j.issn.1001-9391.2018.05.001

深圳市科技研发基础研究项目

本校为第一机构

目的研究邻苯二甲酸二-(2-乙基己)酯(DEHP)对MCF-7细胞的氧化损伤作用,探讨3β-经类固醇脱氢酶(3β-HSD)基因沉默或高表达对DEHP致氧化损伤作用的影响。方法体外培养MCF-7细胞、3β-HSD基因沉默细胞、3β-HSD基因高表达细胞,DEHP对细胞染毒,以MCF-7细胞为对照,不同浓度DEHP(0.00、0.05、0.10、0.20、0.40、0.80 mmol/L)分别染毒24 h,测定3种细胞内氧化损伤指标丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)的变化,荧光定量PCR检测DNA损伤修复基因hOGG1、hMTHl mRNA表达变化, Western blot法检测hOGG1、hMTH1蛋白表达变化。结果DEHP染毒MCF-7细胞后,与对照...

Objective: To study the oxidative damage of di- (2-ethylhexyl) phthalate (DEHP) on MCF-7 cells, and to investigate the effects of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) gene silence or overexpression on DEHP-induced oxidative damage. Methods: MCF-7 cells, 3beta-HSD gene silencing cells and 3beta-HSD gene overexpression cells were treated with different doses of DEHP (0,0.05,0.1,0.2,0.4,0.8 mmol/L) for 24h, then intracellular oxidative damage index such as MDA, SOD, GSH, GSH-PX were detected, DNA repair gene hOGG1, hMTH1 mRNA expression were tested by Q-PCR, hOGG1, hMTH1 protein express...