期刊论文

中文

中华肿瘤防治杂志

1673-5269

2006

13

6

426-429

10.3969/j.issn.1673-5269.2006.06.008

本校为第一机构

目的:探讨凋亡抑制因子Survivin在鼻咽癌组织中的表达及其临床意义.方法:采用逆转录聚合酶链反应(RT-PCR)和Western blot免疫印迹法,检测25例鼻咽癌组织及对应的对侧鼻咽部黏膜、10例正常人的鼻咽部黏膜组织中Survivin mRNA和蛋白的表达.结果:Survivin在正常鼻咽部黏膜组织中不表达.80.0%(20/25)的鼻咽癌组织和40.0%(10/25)的对应对侧相对正常鼻咽部黏膜中均可检测到Survivin基因mRNA和蛋白的表达,Survivin mRNA在鼻咽癌组织中的表达较对应的对侧鼻咽部黏膜组织高,其吸光度(A)比值分别为0.83±0.22和0.29±0.14,差异有统计学意义,P=0.00;Survivin蛋白在鼻咽癌中的表达同样高于对侧鼻咽部黏膜组织...

OBJECTIVE： To study the expression and clinical significance of apoptosis inhibitor survivin in nasopharyngeal carcinoma （NPC）. METHODS： The expression level of Survivin mRNA and protein in biopsies of 25 NPC and opposite nasopharyngeal mucosa and 10 normal nasopharygeal epithelium were examined by RT-PCR and Western blot. RESULTS： No survivin was detected in normal nasopharygeal epithelium. However, the expression of Survivin mRNA and protein were found in 80.0^ （20/25） NPC and 40.0^ （10/25） of opposite nasopharyngeal mucosa,respectiv...