期刊论文

中文

中华肿瘤防治杂志

1673-5269

2005

12

21

1609-1614

10.3969/j.issn.1673-5269.2005.21.003

国家自然科学基金（30271171）； 国家教育部优秀骨干教师基金（教技司2000-65）； 湖南省自然科学基金（01jjy2114）； 湖南省卫生厅科研基金（00022）；

本校为其他机构

目的:利用蛋白质组学方法,识别并鉴定幽门螺杆菌(Helicobacter pylori, H.pylori)处理HepG2细胞后差异表达的蛋白质,为进一步探讨幽门螺杆菌对肝细胞的病理作用机制奠定基础.方法:运用固相pH梯度双向凝胶电泳技术分离H. pylori处理前后HepG2 细胞的总蛋白质,用图象分析软件比较分析,以识别差异表达的蛋白质;应用质谱仪(mass spectrometry)得到相应的肽质指纹图谱(peptide mass fingerprint, PMF),然后搜索数据库鉴定差异蛋白质.结果:鉴定出12种差异表达蛋白质,这些蛋白质包括整合素β-1、蛋白激酶C-α、PINCH蛋白、Ras相关蛋白Rab-37、LIM同源盒蛋白Lhx1、HLA Ⅰ类抗原、血管抑制素和金属蛋白酶...

OBJECTIVE： To investigate the pathological effect of Helicobacter. pylori on human hepatic cells, proteomic methods were used to find and identify differential expression proteins in HepG2 cells that were induced by H. pylori treatment. METHODS： The total proteins of untreated and H. pylori treated HepG2 cells were separated by immobilized pH gradient （ IPG ）-based two-dimensional gel electrophoresis （ 2-DE ）. The differential expression proteins were analyzed by using image analysis software, then identified by using mass spectrometry an...