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ROS-Autophagy pathway mediates monocytes-human umbilical vein endothelial cells adhesion induced by apelin-13

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成果类型:
期刊论文
作者:
Liu, Meiqing;Li, Hening;Zhou, Qun;Zhao, Hong;Lv, Deguan;...
通讯作者:
Chen, Zhe;Li, Lanfang;Chen, Linxi
作者机构:
[Huang, Shifang; Lv, Deguan; Jiang, Jinyong; Tang, Mingzhu; Wu, Di; Liu, Meiqing; Zhao, Hong; Hu, Haoliang; Chen, Zhe; Li, Lanfang; Chen, Linxi; Liu, Jiaqi; Li, Hening; He, Lu; Zhou, Qun; Cao, Jiangang; Wu, Lele] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
[Liu, Meiqing] Second Peoples Hosp Yunnan Prov, Dept Pharm, Kunming, Yunnan, Peoples R China.
[Li, Hening] First Peoples Hosp Yueyang, Dept Pharm, Yueyang, Hunan, Peoples R China.
[Zhou, Qun] Hunan Univ Med, Coll Pharm, Huaihua, Peoples R China.
通讯机构:
[Chen, Z; Li, LF; Chen, LX] U
Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
acetylcysteine;apelin;apelin 13;beclin 1;catalase;hydroxychloroquine;intercellular adhesion molecule 1;rapamycin;reactive oxygen metabolite;reduced nicotinamide adenine dinucleotide phosphate oxidase;reduced nicotinamide adenine dinucleotide phosphate oxidase 4;small interfering RNA;unclassified drug;apelin-13 peptide;reactive oxygen metabolite;reduced nicotinamide adenine dinucleotide phosphate oxidase;signal peptide;animal experiment;animal model;animal tissue;Article;atherosclerosis;atherosclerotic plaque;autophagosome;autophagy;cell adhesion;controlled study;coronary artery tissue;human;human cell;immunohistochemistry;monocyte;mouse;nonhuman;oxidative stress;priority journal;protein expression;THP-1 cell line;umbilical vein endothelial cell;animal;autophagy;cell culture;drug effect;knockout mouse;metabolism;monocyte;physiology;signal transduction;umbilical vein;umbilical vein endothelial cell;Animals;Autophagy;Cells, Cultured;Human Umbilical Vein Endothelial Cells;Humans;Intercellular Signaling Peptides and Proteins;Mice, Knockout;Monocytes;NADPH Oxidases;Reactive Oxygen Species;Signal Transduction;Umbilical Veins
期刊:
JOURNAL OF CELLULAR PHYSIOLOGY
ISSN:
0021-9541
年:
2018
卷:
233
期:
10
页码:
6839-6850
基金类别:
This work was supported by grants from the National Natural Science Foundation of China (No 81270420, 81470434, 81670265, and 81503074), the Construct Program of the Key Discipline in Human Province, the Hunan Province Cooperative Innovation Center for Molecular Target New Drugs Study (Hunan Provincial Education Department document, No 2014405).
机构署名:
本校为第一且通讯机构
院系归属:
药学与生物科学学院
摘要:
Apelin is the endogenous ligand of APJ receptor. Both monocytes (MCs) and human umbilical vein endothelial cells (HUVECs) express apelin and APJ, which play important roles in the physiological processes of atherosclerosis. Our previous research indicated that apelin-13 promoted MCs-HUVECs adhesion. Here, we further explore the mechanism responsible for MCs-HUVECs adhesion induced by apelin-13. Apelin-13 promoted reactive oxygen species (ROS) generation and NOX4 expression in HUVECs. Apelin-13 inducedautophagy, increased proteins beclin1 and LC...

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