期刊论文

Mechanism of radioprotection effects of Astragaloside IV on the liver cell

中文

辐射防护

1000-8187

2017

37

4

309-316

No.81272994:国家自然科学基金 2016JJ2115:湖南省自然科学基金 C2015-17:湖南省卫生计生委科研项目 2015XQD31:南华大学博士启动基金 BKLR2016-01:北京市重点实验室开放基金

本校为第一机构

本文研究了黄芪甲苷(Astragaloside IV,ASIV)对肝细胞放射损伤防护的机制。以γ射线照射L - 02细胞建立辐射损伤模型,分为对照组、黄芪甲苷组(80 μg /mL) 、照射组(4 Gy) 、黄芪甲苷+照射组(80 μg /mL + 4 Gy),于照射前12 h更换含有黄芪甲苷浓度为80 μg /mL的培养基。采用流式细胞术检测黄芪甲苷对照后L - 02细胞早期凋亡率及细胞周期的影响;以罗丹明123荧光探针检测黄芪甲苷对照后L - 02细胞线粒体膜电位改变的影响;免疫荧光法观察γH2AX焦点数;通过Western Blot检测细胞中Nrf2、HO - 1、NQO1的蛋白表达变化。结果显示:照后细胞凋亡率随着药物浓度的增加而降低,黄芪甲苷能有效的缓解电离辐射...

The radioprotection effects and molecular mechanism of Astragaloside IV (ASIV) on the liver cell L - 02 was studied. The experiments were conducted with four groups: the control group,the ASIV group,the irradiation group and the ASIV + irradiation group. The rate of apoptosis and cell cycle were detected by flow cytometry and Rhodamine - 123 fluorescent probe was used to detect potential alternation of the mitochondrial membrane in L - 02 cell. γH2AX foci were detected by immunofluorescence confocal microscopy while the expressions of Nrf2,HO ...