期刊论文

中文

辐射防护

1000-8187

2006

26

2

78-84

10.3321/j.issn:1000-8187.2006.02.003

国家高技术研究发展计划（863）资助项目（编号:2004AA221160）； 国家自然科学基金资助项目（编号:30270423）；

本校为第一机构

应用基因芯片技术分析5 cGy低剂量60Co γ射线照射正常人淋巴母细胞(AHH-1)后基因转录产物水平变化,探讨低剂量辐射对基因表达的影响规律.5 cGy 60Co γ射线照射AHH-1细胞后4 h,提取总RNA,用包含有14 112个基因探针的人类cDNA芯片分析基因转录谱,照射组与对照组细胞的基因表达量差异比值大于2或小于0.5,两次检测结果一致的基因点为有效差异表达基因;用RT-PCR进一步验证部分差异表达基因;Western blot 分析蛋白表达变化.结果筛选出了11个表达上调基因,9个表达下降基因;RT-PCR检测结果与芯片结果相一致,包括Connexin43、BMPR2、NOL6、LOC51760、LYK5等基因;Western blot证实Connexin43基因在翻译水...

In order to investigate the systematic effects of low dose irradiation on transcription regulation of normal human lymphoblastoid cells, cDNA chip containing the probes related to 14 112 human genes was applied to assay the alterations of transcriptional profile of AHH-1 cells exposed to 5 cGy of γ-ray from 60^Co. After a repeat genechip hybridization the genes with 2-fold increase or decrease in expression signal is regarded as a differential expression gene. Then RT-PCR and Western blot were used to confirm the results of genechips. The resu...