期刊论文

中文

中华性传播感染杂志：英文版

1608-6260

2004

02

101-104+135

本校为第一机构

目的建立一种检测临床标本中微量梅毒螺旋体（Tp）的简便、特异、敏感的方法,作为血清学方法的补充诊断梅毒。方法运用双管巢式PcR（SN-PCR）和单管巢式PCR（DN-PCR）扩增了Tp的DNA多聚酶Ⅰ基因（polA）的特异性片段,进行特异性和敏感性试验;用SN-PCR 和DN-PCR检测86份可疑梅毒的临床全血标本中TP polA,同时对相应血清标本用TPPA法检测。结果SN-PCR和DN-PCR polA PCR只在扩增Tp时有特异性产物,灵敏度均约为1个生物。检测86份临床标本,TPPA法阳性62份,DN PCR和SN-PCR PCR阳性数分别为54和 51,结果无显著性差异;TPPA法阴性24份,DN-PCR、SN-PCR阳性数均为5例,。结论SN-polA PCR具有高度敏感、特异、...

Objective: To develop a sensitive, specific and simple method for detection of extremely low numbers of T. pallidum in clinical specimens, as a significant addition to the serologic tests for syphilis diagnosis. Methods: Double-tube nested PCR(DN-PCR) and single-tube nested PCR(SN-PCR) assays were performed to amplify specific fragments of the DNA polymerase I gene(polA) of T. pallidum. Sensitivity and specificity of the two PCR assays were tested. Eightysix whole blood specimens from persons with suspected syphilis were detected by the two nested PCR methods. The TPPA test was used as a compa...