期刊论文

Liu, Pingsheng

英文

ACS Nano

1936-0851

2016

10

3

3312-3322

10.1021/acsnano.5b06852

The authors thank Dr. John Zehmer for his critical reading and useful suggestions. The authors also thank Congyan Zhang and Huimin Na for providing constructions for MLDS and MDT-8/PLIN-1 expression, Wei Ji and Wei Zhao for making Cryo-EM samples and taking the pictures, Dr. Fan Wu for providing liposome samples, Shuoguo Li (Center for Biological Imaging, IBP, CAS) for her help of taking and analyzing SIM images, Shuyan Zhang for making the thin-section samples and taking EM photos, Yanxia Jia (Center for Biological Imaging, IBP, CAS) for assistance with EM and Hongjie Zhang for suggestion to radioisotope experiments. This work was supported by Grant 2011CBA00906 from the Ministry of Science and Technology of China and Grants from the National Natural Science Foundation of China (No. 31000365, No. 61273228, No. 81270932, No. U1402225).

本校为其他机构

The lipid droplet (LD) is a cellular organelle that consists of a neutral lipid core with a monolayer-phospholipid membrane and associated proteins. Recent LD studies demonstrate its importance in metabolic diseases and biofuel development. However, the mechanisms governing its formation and dynamics remain elusive. Therefore, we developed an in vitro system to facilitate the elucidation of these mechanisms. We generated sphere-shaped structures with a neutral lipid core and a monolayer-phospholipid membrane by mechanically mixing neutral lipid...